Sample Preparation for Proteomic Analysis of Barnacle Basal Materials
Abstract
Briefly, this method utilizes sample buffer extraction of barnacle proteins, denaturation, separation via gel electrophoresis based on molecular weight and size. Once vertical separation is achieved, gel image is obtained and protein bands are cut out at the corresponding molecular weights, cutting pattern is recorded, and in-gel digestion using trypsin is performed. This protocol requires a minimum two working days to be completed.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 11, 2023
- Accession Number
- AD1210840
Entities
People
- Christopher M Spillmann
- Dagmar H Leary
- Eric O Mcghee
- William J. Hervey
Organizations
- United States Naval Research Laboratory