Polycaprolactone-collagen Composite Biomaterials for Mandible Regeneration

Abstract

Our primary objective is to demonstrate approaches to create mechanically robust, patient-customized biomaterials forlarge, load-bearing maxillofacial bone defects. To do this, we are developing approaches to integrate a biomoleculedecorated collagen scaffold with micro-scale porosity into a mechanically-robust polymeric frame generated via 3D-printingwith macro-porosity. Through the second year of this project we have completed the design, fabrication, as well asmechanical and in vitro osteogenesis testing of multiple elements of the final composite biomaterial design. We identified a PLA-collagen composite that could support robust mesenchymal stem cell (MSC) viability, osteogenic differentiation, andnew mineral synthesis while rendering it shape-fitting to improve conformal contact with the wound margin. We haveadvanced multiple strategies to incorporate biomolecular signals into the collagen scaffold via transient sequestration,covalent attachment, and through included zinc ions that enhance osteogenic activity. Ongoing efforts are completingbiomolecule incorporation and release experiments to facilitate long-term (>7 day) biomolecule bioavailability via mineralbased factor sequestration chemistries. Our efforts are essential for our goal to identify a shelf-stable, patient customizablebiomaterial that can be seeded with autologous MSCs to regenerate large CMF bone defects.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2023
Accession Number
AD1211457

Entities

People

  • Brandon Harley

Organizations

  • University of Illinois Urbana–Champaign

Tags

DTIC Thesaurus Topics

  • Additive Manufacturing
  • Bone And Bones
  • Bone Diseases
  • Bone Fractures
  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Health Services
  • Manufacturing
  • Materials Science
  • Medical Personnel
  • Osteogenesis
  • Peptide Growth Factors
  • Skull
  • Stem Cells

Readers

  • Immunology and Pathology
  • Nanocomposite Materials Science
  • Trauma Surgery or Emergency Medicine.

Technology Areas

  • Biotechnology