Discovery of Allosteric Modulators of the A3 Adenosine Receptor for Treatment of Chronic Diseases

Abstract

Positive allosteric modulators (PAMs) of G protein-coupled receptors (GPCRs) bind to topographically distinct sites from orthosteric agonists, causing receptor conformational changes that increase agonist affinity, potency, and/or efficacy. 1Himidazo[4,5-c]quinolin-4-amine derivatives were identified as A3 adenosine receptor (A3AR) PAMs. Here, we introduce a 6-step synthesis applied to four groups of 1Himidazo[4,5-c]quinolin-4-amine derivatives to explore structure-activity relationships and pharmacokinetics. We show the ability to fine-tune both 2-cycloalkyl and open ring derivatives as competitive antagonists and/or allosteric modulators. These activities were separated pharmacologically using chimeric mouse/human A3ARs to show the PAM binding region likely to occur at a hydrophobic site on the A3AR cytosolic interface distinct from the orthosteric site. 2-Cyclononyl-N-(3,4-dichlorophenyl) 20 (1 micro M) derivative increased the A3AR agonist potency two-fold in [35S]GTPgammaS binding, as well as Emax (242%). 2-(Heptan-4-yl)-N-(3,4-dichlorophenyl) 2, 2-(hept-4-en-1-yl)-N-(3,4-dichlorophenyl) 10, and 2-(heptan-4-yl)-N-(4-iodophenyl) 31 (1 micro M) derivatives were highly efficacious (Emax = 216%, 241% and, 223%, respectively). Although hydrophobic and having low permeability and high plasma protein binding, derivative 10 was orally bioavailable in the rat. The derivatives tested lacked high-affinity off-target binding to forty-five other membrane proteins. Furthermore, we demonstrated a route for radioiodination at the para-position of a 4-phenylamino substituent to prepare a radioligand for allosteric site binding. Herein, we advanced the allosteric approach to developing drugs for A3AR activation that are potentially event- and site-specific in action.

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Document Details

Document Type
Technical Report
Publication Date
Mar 23, 2022
Accession Number
AD1212219

Entities

People

  • Lucas B. Fallot

Organizations

  • Uniformed Services University of the Health Sciences

Tags

DTIC Thesaurus Topics

  • Alkenes
  • Amino Acids
  • Animal Diseases
  • Blood
  • Cell Membrane
  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Liquid Chromatography
  • Mass Spectrometry
  • Measurement
  • Medical Personnel
  • Organic Chemistry
  • Pharmacology
  • Rodents

Fields of Study

  • Biology
  • Chemistry

Readers

  • Molecular and Cellular Biochemistry
  • Parasitology and Pharmacology of Malaria.