Compressing Time and Space for an In Situ Dermal Graft Printing Paradigm
Abstract
Currently, no studies have investigated excised wound beds as a supportive matrix for a well-defined population of stem cells cultured directly within the wound bed niche. To capitalize on the idea of a wound bed-stem cell synergy, the discovery science phase of work in this proposal will rest on the premise that the native wound microenvironment will be the optimal bioreactor for a stem cell-based dermal graft model. Upon completion of the build of the highresolution MEW process with implementation of a heat-exchange collector plate and establishing fundamental understanding of the collector temperature effect under stationary and single fiber printing modes of operation, optimization of design parameters for the 3D template printing were investigated in Year 2of the project. The systematic design of experiments carried out in the current reporting period yielded significant results, including the quantitative evolution of scaffold morphology with layer number at different collector temperatures. Based on the PI teams established process testbed and understanding of process parameterization in Year 1-2 of the project, a major task for the next reporting period will be to extend the MSC biological phenotype characterization studies for longer duration time courses. Although early indications based on the MSC culture on MEW|0-90o experiments demonstrates an enhancement inpreserving the stem cell phenotype during the first week of culture compared to the 2D controls, the time course will be extended (to two weeks or longer) inthe next design of experiments in order to observe how long the stem cell marker expression is preserved, along with the degree of homogeneity in the 3Dcultured stem cell population. Another major task for the next reporting period will be to investigate the effect of alternate porous microarchitectures (e.g.MEW|0-45o) on the resultant MSC phenotype. Finally, another significant task for the next reporting period will be to demonstrate in s
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2023
- Accession Number
- AD1213950
Entities
People
- Robert C. Chang
Organizations
- Stevens Institute of Technology