Intra-Articular Injection of Alpha-2-Macroglobulin Prevents Post-Traumatic Osteoarthritis
Abstract
Since it is known that cartilage catabolic enzymes in synovial fluid (SF) play a critical role in the onset and progression of post traumatic osteoarthritis (PTOA), the objective of this study is to demonstrate that intra-articular injection of -2 Macroglobulin (A2M),a master inhibitor of these enzymes, would serve as an early therapeutic agent to prevent PTOA after joint injury. In this proposed study, we will test whether early supplemental intra-articular A2M injections will attenuate PTOA pathogenesis 15 weeks after joint injury in the mini-pig model in vivo and we will explore the mechanism how A2M prevents PTOA via blockage IL-1/NF-kb pathway in vitro. In year 3,we published a peer review paper entitled A2M Inhibits Chondrocyte Catabolism by Blocking IL-1/NF-B Pathway on JOR. We have performed 48 pigs surgery with A2M treatment in vivo and have collected all knee joint samples. Currently, we have collected all slides from 48 pigs and are analyzing these samples. Our second paper entitled A Novel Large Animal Model of Posttraumatic Osteoarthritis Induced by Inflammation with Mechanical Stability just accepted by AJTR. We plan to finish the analysis of the data we already collected and write a final report before 2/14/2024. Successful implementation of an injectable therapeutic to prevent the PTOA has the potential to significantly improve the quality of life for thousands of wounded warriors, as well as maximizing their function for return to duty or civilian life.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2023
- Accession Number
- AD1215024
Entities
People
- Lei Wei
Organizations
- Rhode Island Hospital