Investigating Mechanisms of Leukemic Self Renewal in Acute Myeloid Leukemia
Abstract
Leukemia accounts for ~30 percent of pediatric cancer diagnoses. Our lab focuses on AML with rearrangements of the AF10 gene (AF10-R), ahigh-risk subset associated with treatment resistance and disease relapse. A hallmark of 70% of AML cases, including AF10-R, is the dysregulated expression of the HOXA gene cluster and its co-factor MEIS1 (HOX/MEIS). Functionally, HOX/MEIS activation is a critical node in leukemogenesis. It is well established that HOX/MEIS gene expression is sustained by epigenetic regulators that are coopted in leukemogenesis. To comprehensively characterize epigenetic regulators of HOX/MEIS genes, we conducted a phenotypic pooled CRISPR using a custom, high-density domain-focused CRISPR in a MEIS1-GFP leukemia cell line. Our screen uncovered several known and novel candidate regulators of HOX/MEIS expression.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 2023
- Accession Number
- AD1216500
Entities
People
- Karina B. Guerra