Nanovesicle-Mediated Targeting of YAP in Cholangiocarcinoma to Enhance Chemotherapy Sensitivity
Abstract
The goal of this project is to assess the ability to therapeutically target YAP and TAZ mediated cell survival signaling in cholangiocarcinoma. We will utilize milk-derived nanovesicles loaded with siRNA and linked to a targeting aptamer for EPCAM. The purpose is to see if the gene targets can be successfully downregulated through this approach, whether cells and/or tumor models are less viable when treated with the nanovesicles, and whether this nanovesicle treatment also improves the response to standard chemotherapy treatments. The effects will be evaluated in cell lines models and in vivo mouse models. The overall research objective is to evaluate the therapeutic efficacy of nanovesicle based small-interfering RNA targeting YAP/TAZ as a single agent and in combination with a standard of care chemotherapy regimen: gemcitabine and cisplatin.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2023
- Accession Number
- AD1216867
Entities
People
- Jack Sample
- Mincheng Yu
- Rory L Smoot
- Shohei Takaichi
- Tushar Patel
Organizations
- Mayo Clinic