Engineering Artificial Metalloenzymes for Selective Catalysis in Complex Media
Abstract
The originally stated goals of this proposal were as follows: 1. Develop immobilization-based evolution approaches to optimize dirhodium artificial metalloenzymes (ArMs) for selective C-H functionalization in complex aqueous solutions. 2. Evolve ArMs containing base metal cofactors that catalyze selective C-H functionalization of representative drug molecules and pesticides in aqueous solutions. 3. Develop cofactor activation approaches that allow the use of chemically inert cofactors to form reactive ArMs only upon scaffold activation. Progress toward these goals was significantly impacted by my groups move to Indiana University during the first funding period and by COVID-19 related university shutdowns and lab occupancy reductions. This report covers research conducted under the original award made while I was at the University of Chicago (18-1-0034) and following transfer of this award to Indiana University (19-1-0074). Despite these challenges, we were able to make significant progress on all three of the originally proposed goals. We also expanded the scope of the first goal to include ruthenium-based ArMs and to pursue initial studies on ArMs containing genetically-encoded bipyridyl alanine residues.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 14, 2021
- Accession Number
- AD1217574
Entities
People
- Jared C Lewis
Organizations
- Indiana University