Influence of Substrate Material on the Composition and Diversity of Bacterial Cultures Grown from Human Dental Biofilm Specimens Ex Vivo
Abstract
Titanium and yttria stabilized zirconia oxide are materials commonly found at the soft-tissue interface surrounding dental implants. The influence of these surfaces on the processes of bacterial adhesion and biofilm development may affect clinical performance and patient susceptibility to inflammatory peri-implant disease. The purpose of this study was to evaluate the influence of the substrate material on biofilm composition and complexity. We cultured biofilms on hydroxyapatite (HA), TI, and YSZ surfaces using dental plaque specimens obtained from three human donors. Average roughness (Ra) values of all surfaces approximated 0.2 microns. Duplicate cultures grew for one, two, three, six or nine days. We then analyzed biofilm composition and diversity using 16S rRNA sequencing. The Shannon Diversity Index (SDI) was calculated for each experimental group, using a linear mixed-model analysis to test for statistically significant differences. Microbial profiles were inter-compared in a pairwise fashion to establish dissimilarity scores, which were recorded in a distance dissimilarity matrix. Biofilms grown on HA surfaces exhibited significantly higher alpha diversity compared with those formed on TI or YSZ (p<0.0001). However, relative abundances of the predominant phyla and genera did not appear to be different across experimental groups. Under the conditions described, biofilms grown on TI or YSZ appeared significantly less complex than those formed on HA. Limited evidence suggests that high biofilm diversity may be associated with oral health. Thus, compared with periodontal health, peri-implant health may be more difficult to maintain, due in part to characteristics of the implant/abutment material.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 23, 2022
- Accession Number
- AD1222395
Entities
People
- Joseph K. Retrum
Organizations
- Uniformed Services University of the Health Sciences