microRNA of Circulating Exosomes for Systemic Sclerosis
Abstract
The purpose of the studies performed under the support of this grant was to identify novel and sensitive noninvasive biomarkers for the diagnosis and assessment of the severity and extent of Systemic Sclerosis (SSc) tissue fibrotic involvement. To accomplish this goal we employ a quantitative PCR assessment of the levels of specific miRNAs contained within circulating exosomes isolated from the serum of SSc patients. Three cohorts are included in the study: healthy donors and SSc patients with and without SSc associated pulmonary fibrosis. We expect that these miRNA biomarkers to be identified in this project may be used as quantitative and unbiased parameters of SSc tissue fibrosis and as indicators for the selection of effective disease treatment based on relevant SSc physiopathologic processes. A total of 142 serum samples from SSc patients and normal subjects that were maintained frozen at the Scleroderma Center serum bank were used to isolate exosomes and the exosome samples were used for further isolation of total RNA, and for synthesizing first strand miRNA cDNA. PCR was performed with 96 cDNA samples and analysis of the results and statistical evaluation are presented.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2023
- Accession Number
- AD1223164
Entities
People
- Sergio Jimenez
Organizations
- Thomas Jefferson University