Development of Novel Modeling Approaches for Rapid Risk Characterization of PFAS and PFAS Alternatives
Abstract
Per- and polyfluoroalkyl substances (PFAS) are persistent and pervasive chemicals frequently encountered by humans and wildlife. As a chemical class, PFAS include some 4000 different compounds making it difficult to adequately assess human risks due to PFAS exposure. As such, there are several data gaps, which often cannot be filled through traditional means for assessing exposure outcomes such as two-dimensional (2D) tissue culture on plastic or in vivo models. Organ-on-a-chip models and other full tissue models have become widely adopted as more accurate means for predicting human outcomes in response to exposure to drugs or compounds of interest. In this study, Emulate (Emulate, Inc.; Boston, MA) liver and renal proximal tubule chips were exposed to various concentrations of perfluoro octane sulfonic acid (PFOS) or perfluoro hexane sulfonate (PFHxS) over time. Cytotoxic effects, which were different from those observed in 2D tissue culture, were detected because of exposures to both compounds. Phenotypes that may be detrimental to organ health after exposure to PFOS or PFHxS were also identified, such as changes in uric acid, albumin production and reactive oxygen species and reactive nitrogen species production compared with untreated chips. PFOS and PFHxS were detected in cell lysates and medium effluent over time.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 20, 2024
- Accession Number
- AD1224232
Entities
People
- Allison E. Melka
- Elizabeth S Dhummakupt
- Jennifer R. Horsmon
- Priscilla E. Lee
- Tyler D. Goralski
Organizations
- United States Army Combat Capabilities Development Command