Amplification Events Altering Tumor Microenvironment That Drive Metastasis in HER2+ Breast Cancer
Abstract
After several rounds of failed knockout/knockdown attempts we have now had a company build the knockdowns for the various constructs. We have knockdowns of three genes and have observed compensatory gene expression results in other related genes. We have also constructed overexpression constructs as a backup in case these knockdowns did not work and they can be used in rescue experiments. A bioinformatic screen to inhibit the pathways through alternative approaches has revealed several potential drugs that could be used to inhibit the pathways in tissue culture. Initial tissue culture results into growth rate and morphology are being repeated to confirm potential results.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2024
- Accession Number
- AD1225094
Entities
People
- Eran R Andrechek
Organizations
- Michigan State University