Modeling Lupus Nephritis in a Human Kidney Microphysiological System
Abstract
The kidney is highly susceptible to injury from many systemic inflammatory and autoimmune diseases, including systemic lupus erythematosus (SLE), which is one of the FY19 CDMRP Topic Area. Among people with SLE, kidney involvement (lupus nephritis) affects 60%-80% of SLE patients and is recognized as a main contributor to morbidity and mortality. End stage renal disease occurs in ~25% of SLE patients within 15 years of onset, even with modern day immunosuppressive treatment. We have developed a three-dimensional flow directed kidney-on-a-chip populated with human kidney cells, with functional characterization of key component structures of the kidney cortical tubulo-interstitium. In Year 2, we continued our work from Year 1, and focused on major task 1 and major task 2, and have resolved the technical hurdles in the matrix variability to faithfully recapitulate kidney microvessel on a chip. Our overall goal is to use these pre-clinical studies to discover more effective strategies for treating and curing individual patients with SLE, so that clinical studies can then be conducted to improve their quality and quantity of life.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 2023
- Accession Number
- AD1225095
Entities
People
- Jonathan Himmelfarb
Organizations
- University of Washington