Elucidating Early Events in HGSC Pathogenesis: A Single-Cell Multiomics Approach to Robustly Trace Cell Lineage, Clonality, and Phenotypes of TP53- Mutated Cells
Abstract
The purpose of this project is to characterize lineage relationships within epithelial cells of the fallopian tube using single cell assays leveraging mitochondrial DNA mutations to determine clonality. Better understanding the cell makeup and lineages in the fallopian tube may give insight into the early stages and initiation of tubo-ovarian high-grade serous carcinoma. To accomplish this we are collecting fresh human fallopian tube, ovarian, and endometrial tissues and profiling the cells at the single cell level to simultaneously assess cell states and clonality. To date, we have profiled 6 samples from 2 patients using mitochondrial-single cell ATACseq, with the computational analyses and additional sample collection/processing underway. We have been technically successful in obtaining and processing the samples, acquiring high quality sequencing data. The significance of the results and final conclusions will be better understood with the additional samples and more in-depth computational analyses.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 2023
- Accession Number
- AD1225990
Entities
People
- Ansuman Satpathy
- Brooke Howitt
- Caleb A Lareau
Organizations
- Stanford University