Supercooled Ex-Vivo Porcine VCA Preservation to Extend the Timeline Between Procurement and Transplantation and Enable Tolerance Induction to Eliminate Immunotherapy Needs and Risks
Abstract
The project aims to develop a novel technology to preserve vascular composite allografts for extended periods. This project uses a porcine model. Through this project, we developed a novel protocol to perfuse porcine limbs for 24h, allowing successful transplantation. Next, we aimed to extend the preservation period at 48hto match the time needed for immune tolerance protocols using mixed chimerism. We tested sub-zero non-freezing (supercooling)of porcine hindlimbs and compared it with controls. We demonstrated successful supercooling at -5C for 48h, and assessed the recovered limbs with normothermic machine perfusion. The perfusion parameters were improved by supercooling but more optimization of the metabolic outcomes are needed before in vivo applications.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 2023
- Accession Number
- AD1226548
Entities
People
- Korkut Uygun
Organizations
- Massachusetts General Hospital