Hyperbaric Oxygen Therapy to Mitigate Childhood Radiation-Induced Neurocognitive and Skeletal Toxicity: Multimodality Evaluation in a Young Rodent Model
Abstract
The purpose of this grant is to test the hypothesis that hyperbaric oxygen therapy (HBOT) can ameliorate or prevent the damaging effects of radiation therapy (RT) used to treat cancer in pediatric subjects. Effects of HBOT will be tested on the musculoskeletal (MSK) system and on cognitive abilities in mice. Hindlimbs are being assayed with CT and with physical measurements. The brain is being examined with multimodal imaging, assays for molecular biomarkers, neuropathological analyses, and multiple behavioral assays. Results thus far indicate the following: 1). Mice can tolerate HBOT (20 sessions: M-F, 90 min each for 4weeks, 2 ATM) and hindlimb RT (20Gy, 1 fraction); 2). After 6 months of follow-up after RT there are no significant MSK changes. 3). On longer follow up we are beginning to see MSK changes, thus we will continue to follow the mice until we get to the primary end point of MSK injury. We will then be able to evaluate the protective effects of HBOT. 4). We have detected early RNA seq and serum cytokine biomarker expression after RT.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 2023
- Accession Number
- AD1226600
Entities
People
- Craig Weiss
- John Kalapurakal
Organizations
- Northwestern University