Liquid Biopsy Detection of Structural Variant Breakpoints to Monitor Ovarian Cancer Clonal Evolution: A Pilot Study
Abstract
This project aims to develop a platform to map and monitor HGSOC sub-clonal evolution by integrating whole genome sequencing (WGS) of tumor biopsies and the detection of structural variants (SVs) by cell-free DNA (cfDNA)-based approach. The major achievements regarding tumor biopsies: multi-site whole genome sequencing on pre-treatment HGSOC biopsy specimens from two cases has been successfully performed. Confident calls of SVs have been determined by multiple methods. Reconstruction of sub-clonal by SVs and identification and validation of SVs are the priorities. Modification of current protocol to enroll prospective cases has been approved. The WGS of multi-site biopsies from the first case met the criteria is ongoing. The significant progress has been made to streamline workflows of digital droplet PCR assays for detection of SVs from synthetic cfDNA and determine the analytical parameters. Assays to consistent isolation of cfDNA from plasma is established.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 2023
- Accession Number
- AD1227415
Entities
People
- Robert T Hillman
Organizations
- The University of Texas MD Anderson Cancer Center