Using Bioinspired Next-Generation Cryoprotectants to Advance Ex Vivo Preservation of Vascularized Composite Allografts at High Subzero Temperature
Abstract
Advancements in tissue transplantation are tightly constrained by the limited preservation time of vascular composite allografts. Innovative biopreservation solutions combined with subzero storage protocols can allow and demonstrate the short-term suspended animation of extremity grafts. Our objective with this grant was to apply X-Thermas scaled-up product (XTViVoTM) intended for sub-zero organ preservation and explore its advanced preservation capabilities to extremities such as whole rat forelimbs and swine hind limbs for up to 72h periods of ischemia resulting in full restoration of form and function posttransplant. Based on this reporting period, we have continually instituted and maintained scale-up practices at X-Therma within our new cleanroom and facility, successfully devised to produce thea method to scale up production of the biomimetic peptoid at a 100g scale,, formulate it within the XT-ViVo(registered) preservation solution at a multi-liter scale adequate for all in vivo studies. The final XT-ViVo(registered) solutions meet stringent quality requirements and pass robust release testing criteria. With our XT-ViVo(registered) formulation, our partners at JHU have successfully preserved the limbs of a total of 35 animals. Analysis included typical parameters including (1) macroscopic observation, (2) external skin regeneration, (3) nerve regeneration, (4) overall grip strength and (5) overall inflammation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 2023
- Accession Number
- AD1227495
Entities
People
- Byoung C. Oh
- Gerald Brandacher
- Mark Kline
Organizations
- Johns Hopkins University