Characterization of the Effects of Mucins on the Growth and Biofilm Formation of Pseudomonas Aeruginosa and Analysis of Cleaved Mucin Glycan Structures
Abstract
This report presents research conducted to characterize the effects of mucins on Pseudomonas Aeruginosa (PA01) cell growth and cell adhesion and also analyzed the cleaved glycan structure. We showed that the protein profiles and glycosylation extent of natural mucus and industrial mucins changed significantly after high heat denaturation by autoclave and these denatured mucins supported the growth of PA01, but had no effects on the cell adhesion that leads to biofilm formation. On the other hand, the native mucins, mucin IIf and mucin IIf, sterilized by filtration exhibited inhibition of cell adhesion; however the presence of native mucins had no effects on cell growth. Furthermore, the cleaved glycan structures were also analyzed using Mass spectrometry and ATRIR. Our results support the hypothesis that native mucins with intact glycans interact with bacterial cells, resulting in less biofilm formation. These findings show promise for in vivo inhibition of biofilm formation associated with wound infection and sepsis.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 08, 2024
- Accession Number
- AD1227842
Entities
People
- Jinny L. Liu
- Keith E Whitener
- Mathew Thum
Organizations
- United States Naval Research Laboratory