NRF2 Pathway Activation in the Lung Tumor Microenvironment Macrophages
Abstract
Lung is physiologically populated by numerous immune cells, that can be skewed to support the growth of tumor cells. My main goal is to understand how cancer cells interact with the surrounding environment, creating an immunosuppressive tumor microenvironment, to promote growth. This knowledge can ultimately render new vulnerabilities that can be addressed by the development of new drugs or repurposing of known drugs, eventually leading to an increase in survival of lung cancer patients. Bone marrow derived macrophages (BMDM) from A/J mice show an increased expression of NRF2 and HO-1 upon stimulation with conditioned media from lung cancer cells, this increase is accompanied with increased expression of CD206, arginase, IL10 and others, showing the ability of cancer cells to skew macrophages towards a tumor promoting phenotype. Moreover, CD11 and CD3 cells were isolated from A/J mice injected with vinyl carbamate (or saline for control group). Both CD11 and CD3 cells, showed an increased expression of HO-1 2 weeks after vinyl carbamate injection. These data suggest that NRF2->HO-1 pathway is activated in macrophages early in the progression of lung cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2024
- Accession Number
- AD1229235
Entities
People
- Ana S. Mendes Leal
Organizations
- Michigan State University