Ultrasound to Diagnose and Treat Heterotopic Ossification
Abstract
In this project, we investigated Doppler ultrasound imaging and therapeutic ultrasound histotripsy for the diagnosis and treatment of acquired heterotopic ossification (HO). We found that we could use Doppler ultrasound parameters within FDA limits to enhance twinkling on minerals in both ossified cells and in the murine model of acquired HO. On Day 19 in cell-deposited minerals, twinkling correlated well (0.78) with the percent area mineralized and the number of minerals; correlation decreased on Days 22 and 25. In mice, twinkling was increased by 6 percent on Day 6 in the HO compared to contralateral controls (p=0.06), and continued to increase to up to 23 percent on Day 15 (p=0.07). The manuscript on these results will be submitted this summer. On the treatment side, we found that ultrasound histotripsy at 1.07 MHz with acoustic pressures of 17 MPa positive and 6 MPa negative (0.1 ms pulses repeated every 5 ms for 4 min) successfully fractionated ossified cells with minimal damage to the surrounding cells and tissue-mimicking phantom. Murine studies to treat HO showed the ultrasound treatment may have slowed the development compared to sham and, excitingly, did not increase the risk of recurrence. A manuscript on treating HO in ossified cells and mice will be submitted for publication this summer. These results demonstrate the promise of ultrasound to diagnose and treat early HO; however additional studies are warranted prior to clinical translation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 2024
- Accession Number
- AD1229681
Entities
People
- Julianna Simon
Organizations
- Pennsylvania State University