Hydrogel Preparation and 3D Cell Assay with no Flow Days 1-3, then Flow for Days 4-10
Abstract
Fibrinogen: Make a 15mg/mL stock of fibrinogen in DPBS in a 37 degree C water bath. It will take about 1tilde 2 hour to completely dissolve. [Fibrinogen doesn't dissolve in water.] DO NOT VORTEX. The solution will be hazy. Freeze in 1mL aliquots at -80 degree C. [Do not filter yet.] Aprotinin: Dissolve 1mg of Aprotinin in 160uL of DPBS for a concentration of 6.25mg/mL. Dispense into 20uL aliquots and freeze at -80 degree C. [Do not filter yet.] Avoid freeze-thaws. Thrombin: Reconstitute in DPBS at 100U/ml. Store in 100uL aliquots at -80 degree C. rhVEGF: Reconstitute in 1 percent BSA at 50ug/mL. rh Angiopoietin-1: Reconstitute in 1 percent BSA at 10ug/mL.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 29, 2019
- Accession Number
- AD1230785
Entities
People
- Christina E. Zook
Organizations
- Massachusetts Institute of Technology