Genetic and Biochemical Studies of Drug Resistance in Pathogenic Bacteria.
Abstract
Sixty strains of Pseudomonas aeruginosa were selected from clinical stock cultures and used as donors of drug resistance to examine the presence of R factors. They were highly resistant to one or more of six drugs including tetracycline(TC), chloramphenicol(CM), streptomycin (SM), sulfanilamide (SA), carbenicillin (CPC), and kanamycin (KM). Three FP(-), auxotrophic mutants of P. aeruginosa were used as recipients, which had been made resistant to either rifampin (RMP) or KM by in vitro mutation. Each donor was mated with each of the three recipients, and 45 strains were found to transfer their resistance to one or more recipients. All of these R factors could not be transmitted to other species belonging to the family Enterobacteriaceae. Cultures of clinical origin Providencia strains were resistant to various aminoglycoside antibiotics including lividomycin and the gentamicin C complex but sensitive to kanamycin A and a new kanamycin derivative, BB-K8. The antibacterial activity of sisomicin (SS), a new aminoglycoside antibiotic active toward clinical isolates of Pseudomonas aeruginosa, was determined and compared with that of the gentamicin C complex.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 06, 1974
- Accession Number
- ADA002796
Entities
People
- Susumu Mitsuhashi