Cultivation of Hepatitis Virus in Tissue Culture.
Abstract
The author's major efforts have been devoted to attempts at cultivation of Hepatitis B virus by using 'slow virus techniques'. By means of co-cultivation, mixed monolayers of normal hepatocytes were co-cultivated with hepatocytes obtained from liver biopsies of patients with Hepatitis B Surface Antigen positive chronic aggressive hepatitis. The cell layers which resulted are being maintained for long periods and are also being sub-cultured for evidence of either cytopathogenic change or for evidence of multiplication of Hepatitis B Surface Antigen as determined by the concentration of antigen in serial pour offs as assayed by the radioimmunoassay. Similarly, feeder layers have been established using chronic active hepatitis liver cells as the feeder source and primary human hepatocytes as the feeder layer. Feeder layers are also being formed using other sensitive tissue culture lines including human diploid lung cells (WI 38) and Hela cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 24, 1974
- Accession Number
- ADA002998
Entities
People
- G. L. Gitnick
Organizations
- University of California, Los Angeles