Oxygen Toxicity in the Mammalian Brain

Abstract

The lactate dehydrogenase (LDH) activity of mouse brain homogenates was examined after exposure to hyperbaric oxygen (5763.8 mm Hg PO2) and compared to room air controls (158.8 mm Hg PO2). The effect of reduced glutathione on LDH activity after hyperbaric oxygen exposure was also examined. The activity of LDH after treatment with hyperbaric oxygen was significantly diminished when compared with controls. In the presence of reduced glutathione, homogenates exposed to hyperbaric oxygen demonstrated higher activity than did homogenates incubated without glutathione. It is concluded that oxygen induced inhibition occurs through the oxidation of essential free sulfhydryl groups and that this oxidation can either be prevented by reduced glutathione or the disulfide bridges may be reduced to free sulfhydryl groups by the glutathione after oxidation.

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Document Details

Document Type
Technical Report
Publication Date
Dec 01, 1974
Accession Number
ADA003229

Entities

People

  • Dennis A. Baeyens
  • Joseph O. Bonnett

Organizations

  • United States Army Aeromedical Research Lab

Tags

DTIC Thesaurus Topics

  • Aviation Medicine
  • Biological Sciences
  • Biomedical Research
  • Central Nervous System
  • Classification
  • Free Radicals
  • Incubation
  • Inhibition
  • Metabolism
  • Molecules
  • Nervous System
  • Oxidation
  • Partial Pressure
  • Protective Agents
  • Security
  • Toxicity

Readers

  • Marine Mammal Biology
  • Mathematics or Statistics
  • Molecular and Cellular Biochemistry