Cryop Reservation of Human Granulocytes.
Abstract
The authors describe a method compatible with component blood banking for the harvesting and freezing of buffy coat leukocytes. It simplicity suggests that application of the cryotechnique employed will be adaptable to large numbers of cells taken in multiple units from single donors. The principle of the method is sedimentation of buffy coat leukocytes with hydroxyethyl starch (HES) to aggregate erythrocytes and concentrate the leukocytes, and cryopreservation by cooling the concentrate to -80C in the presence of residual HES and 5% dimenthylsulfoxide (DMSO).
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 15, 1974
- Accession Number
- ADA003585
Entities
People
- Fabian J. Lionetti
- Joel M. Gore
- Stephen M. Hunt
- William A. Curby