Reproduction of Venezulean Equine Encephalomyelitis Virus at Low Ionic Strength
Abstract
A study was carried out of the effect of low ionic strength on the reproduction of Venezuelan equine encephalomyelitis (VEE) virus. Data obtained showed that low ionic strength inhibits in reverse the process of virus release from the cell at all stages of virus reproduction. An examination was carried out of the kinetics of the virus escape from cells with a change of ionic strength and the possibility of employing low ionic strength to accumulate intracellular virus in large quantities. It was established that under hypotonic conditions synthesis of viral RNA and proteins in an infected cell occurs at a normal rate. An analysis was carried out of viral structures formed in cells in a normal and hypotonic state. It was shown that with low ionic strength in a logarithmic period viral ribonucleoproteins formed and accumulated in the cells which were probably bound with membranes slower than under normal conditions.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 28, 1975
- Accession Number
- ADA006206
Entities
People
- F. I. Yershov
- I. B. Tazulakhova
- S. S. Grigoryan
- T. M. Sokolova
Organizations
- United States Army Medical Research Institute of Infectious Diseases