Establishment of an 'in vitro' Model of Dengue Infection for the Study of Immunologic Events in this Disease
Abstract
Pathologic studies have shown that dengue virus replicates in organs rich in lymphocytic tissues. Studies in vitro with monkey lymphocytes demonstrated that viral replication is dependent upon blast transformation. Therefore the replication of dengue-2 virus in three human lymphoblastoid cell lines of B cell characteristics in continuous culture (Raji, 8866, Wil 2WT) and in human peripheral lymphocytes (HPL) stimulated by mitogens was studied. Dengue-2 virus (SEATO strain) readily replicated in the three human lymphoblastoid cell lines without apparent effect on the host cells. Persistently infected cells produced virus for at least five months. The kinetics of the infection and the localization of the dengue antigens were studied by immunofluorescence and electron microscopy. Persistently infected Raji cells expressing viral antigens on their surface formed rosettes with normal human T lymphocytes and bound human platelets in the presence of human antibody to dengue virus. Immune complexes in sera of patients with dengue could be demonstrated and serum enzyme inhibitors were depressed.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 25, 1974
- Accession Number
- ADA006220
Entities
People
- Argyrios N. Theofilopoulos
Organizations
- Scripps Research