A Study of the Factors Affecting the Cultivation of Malaria Parasites 'in vitro'.
Abstract
The erythrocytic stages of Plasmodium knowlesi grown through one cycle in vitro contain less RNA than equivalent-stage parasites isolated directly from the host (Trigg and Gutteridge, 1972). This conclusion was drawn from direct chemical measurement of the RNA content of bulk cultures of P. knowlesi and supported by indirect evidence on the incorporation of radioactive precursors into DNA and RNA fractions and on the microdensitometric measurements of parasites strained histochemically for RNA. A study has therefore been made of the RNA species synthesized in P. knowlesi to determine whether the parasite is dependent on its host for RNA and to determine whether RNA synthesis occurs throughout the whole of the asexual cycle in the blood.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1975
- Accession Number
- ADA006591
Entities
People
- Peter I. Trigg
- Petter G. Shakespeare
Organizations
- National Institute for Medical Research