Mechanism of Action of Antigens.
Abstract
Studies on the chemical composition and membrane enzyme activities of lymphocytes from the thymus and spleen of the ACI and F344 strains of genetically inbred rats showed that there are not genetic differences between unimmunized animals of these strains. Techniques of solubilizing membrane immunoglobulins with nonionic detergents have been developed, and 1% Triton X-100 was selected as the standard method of solubilizing membranes. In using this new technique, the presence of IgM and IgG on the splenic and thymic lymphocytes of both immunized and unimmunized animals was demonstrated. This technique is also being applied to the isolation of histo-compatibility antigens. Studies on T-cell markers have progressed to the point of having developed a rabbit anti-rat brain serum (anti-theta serum) which reacts with 100% of thymic lymphocytes by indirect immunofluorescence. There are at least two, and possibly three, thymus-specific antigens of molecular weights 65,000 and 25,000 daltons.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 16, 1975
- Accession Number
- ADA008037
Entities
People
- Charles T. Ladoulis
- Thomas J. Gill Iii
Organizations
- University of Pittsburgh