Enzyme Immobilization Alternatives for the Enzyme Alarm.
Abstract
The research concerns the study of alternative processes for coupling the cholinesterase enzyme to various supports in order to achieve enzyme-support product(s) which can be produced in large quantities, stored and then provide at least 12 hours of reliable operation after deployment. Since prior work has emphasized physical entrapment of the enzyme as means of immobilization, this project is oriented primarily to chemical bonding and cross-linking processes. The highest enzyme activity derivatives achieved to date are cholinesterase attached to glass beads by azo coupling and glutaraldehyde cross-linking. Although the measured activities are high, their thermal stabilities are unacceptable.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 1975
- Accession Number
- ADA009764
Entities
People
- Evelyn S. Erenrich
- Herman W. Levin