The Development of a Biological Test System for Calibrating and Evaluating the Performance of Computer-Controlled Optical Scanners
Abstract
A method was developed for processing human red blood cells (RBCs) for microstereology analysis of major cell dimensions. Using this preparative method, RBCs from five normal human donors were prepared for thin section electron microscopy. A test film containing 250 electron micrographs of human RBCs was prepared and analyzed by microstereology methods. Using these methods, the calculated RBC mean corpuscular volume (MCV) is 79.6 cubic micrometers and the mean RBC surface area is 130.1 square micrometers. These results are consistent with results obtained by other quantitative microscopy techniques. The film is of high quality and preliminary testing indicates that the film is suitable for use in evaluations of computer soft ware that is designed to control computer-controlled optical scanners that are utilized for boundary tracing in biological materials.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 1975
- Accession Number
- ADA011579
Entities
People
- Jonathan S. Wallach
- Michael G. Cedars
- Ronald S. Weinstein