Intrinsic and Chemically Produced Microheterogeneity of Staphylococcus aureus Enterotoxin Type C

Abstract

Staphylococcus aureus enterotoxins C sub 1 (sec sub 1) and C sub 2 (sec sub 2) produced from 50-liter quantities of crude culture supernatants were purified chromatographically in a neutral or acid milieu. Microheterogeneity of sec sub 1 was markedly increased by treatment of the purified toxin with alkali, and new, more acidic charged species appeared. Sec sub 2 was more heterogenous than any of the other S. aureus enterotoxins and was affected only slightly by treatment with alkali. Prolonged incubation of the organism during production of the sec sub 2 produced changes in charged species that may be related to a bacterial deamidase, since similar changes were not seen with alkaline treatment of the purified toxin. Although sec sub 1 and sec sub 2 showed complete identity immunologically, they are separate, distinct toxins, and alkali treatment of sec sub 1 did not produce sec sub 2.

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Document Details

Document Type
Technical Report
Publication Date
Feb 13, 1975
Accession Number
ADA013704

Entities

People

  • Anna D. Johnson
  • Joseph F. Metzger
  • Leonard Spero

Organizations

  • United States Army Medical Research Institute of Infectious Diseases

Tags

DTIC Thesaurus Topics

  • Biochemistry
  • Biological Toxins
  • Cellulose
  • Chemical Properties
  • Chemistry
  • Fermentation
  • Gel Electrophoresis
  • Heterogeneity
  • Immune Serums
  • Infection
  • Intramuscular Injections
  • Materials
  • Molecular Weight
  • Physical Properties
  • Production
  • Staphylococcus Aureus
  • Uss Valley Forge

Readers

  • Civilian Systems Systems Program Capability Development and Upgrade Support Activity Expense and Pay Management.
  • Materials Science and Engineering.
  • Microbial Pathology