Investigations of Attenuated Strains of Group A Arboviruses.
Abstract
This is a study to develop attenuated strains of Eastern Equine Encephalitis (EEE) Virus and Western Equine Encephalitis (WEE) Virus which would be suitable candidates for human vaccines. The clone 15 strain of WEE virus has been utilized to prepare a chicken embryo cell culture vaccine as a candidate for human use. About 150,000 doses of Final Vaccine have been prepared and stored in bulk. About 3,500 doses have been lyophilized as the Final Packaged Vaccine. Preliminary studies on the purity, potency, and safety of the vaccine indicate that 10 to-the-8th to 10 to-the-9th power logs of virus does not produce clinical disease in monkeys, guinea pigs, or weanling mice when inoculated intracerebrally and systematically. Studies indicate that the vaccine is free of adventitious agents including anaerobic and aerobic bacteria, fungi and murine viruses. The vaccine does produce two plaque types when cultured in the chicken embryo cell system. Studies have been initiated to develop an attenuated vaccine candidate strain of EEE virus, using the BeAn 221 isolate. Studies with mutogenic chemicals and serial passage studies are in progress in an attempt to isolate a virus which has lost its neurotropic characteristics. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 1975
- Accession Number
- ADA014992
Entities
People
- J. V. Formica
- Philip H. Coleman
Organizations
- Virginia Commonwealth University