Investigation of E. coli Enterotoxins.
Abstract
In the course of investigating E. coli enterotoxins, it was discovered that trypsin treatment of partially purified enterotoxin from strain H197 (078:H11) as well as cell-free culture filtrates from several different enterotoxigenic strains resulted in a 4- to 20-fold increase in heat-labile rabbit skin vascular permeability activity (PF). PF assays performed on culture filtrates after short-term incubation in vivo (i.e., in ligated rabbit ileal loops) did exhibit elevated PF titers compared with uninoculated controls. These findings are consistent with the hypothesis that E. coli enterotoxins represent a new class of protoxins and as such may undergo in vivo enzymic modification in order to become fully pathogenic. The details of findings with cell-free culture filtrates as well as preliminary purification studies with trypsin-treated enterotoxin are presented in this report.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1975
- Accession Number
- ADA015076
Entities
People
- Ruth Rappaport