In Vitro Reactivity of 3-M KC1-Solubilized Murine Histocompatibility (H-2) Antigens
Abstract
It is well established that when allogeneic cells are cultured together in vitro they give rise to stimulation and blast transformation (the MLC). The mechanisms and genetic basis for this reaction have been a matter of intensive investigation. It was thought that the major histocompatibility HL-A antigens in man and H-2 antigens in the mouse were mainly responsible for this reactivity. However, recent evidence has demonstrated the existence of MLC reactivity between cells from mice belonging to the same H-2 allele. These studies have led to the definition of the M locus which is not linked to the major histocompatibility locus. In addition, MLC reactivity has also been demonstrated between mice differing at the thy. 1 marker, and between mice with L superscript d differences. Although humoral immunity has been associated with the major H-2 loci, a recent study has shown that non H-2 antigen can generate strong antibody response. These investigations were carried out to study the ability of solubilized extracts of mouse lymphoid cells to act as antigenic determinants of histocompatibility low in various in vitro assays of cellular immunity.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1974
- Accession Number
- ADA015407
Entities
People
- Amandeep Kaur
- D. M. Strong
- K. W. Sell
- R. C. Knudsen
- W. E. Vannier
Organizations
- Naval Medical Research Center