The Nature of the Reaginic-Like Response in Infected Mice.
Abstract
The induction of antibody by spleen cells in culture after primary exposure to an antigen, mouse crythrocytes (RBC) parasitized with the NYU-2 strain of Plasmodium berghei, has been demonstrated by the Cunningham modification of the Jerne plaque assay technique. This finding corroborates the indirect evidence of Hirsch and Plescia who reported that T cells taken from mice four hours after immunization with sheep RBC can stimulate normal spleen cells to induce specific antibodies in vitro. In the present experiments, the observed hemolysis resulting in the formation of a plaque is interpreted as being the result of a specific antibody-antigen reaction. Support for this premise lies in the observation that the hemolysis is complement-dependent and can be inhibited by cycloheximide which is ineffective in suppressing cytophilic antibody formation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 31, 1975
- Accession Number
- ADA016652
Entities
People
- Saul Malkiel