Specificity and Immunosuppressive Potency of a Rabbit Antimouse T Cell- Specific Antiserum
Abstract
An attempt was made to prepare a specific heterologous rabbit antimouse T cell antiserum (anti-MTLA) by absorbing rabbit antimouse thymocyte globulin (ATG) with spleen cells from BALB/c T sub X B sub M mice. Cytotoxicity data showed that whereas ATG was cytotoxic to both T and B cells, anti-MTLA was highly cytotoxic to only T cells. Whereas spleen cells treated with ATG and complement (C) failed to respond in all assays studied, spleen cells treated with anti-MTLA and C: (1) responded to the B cell mitogens but failed to respond to the T cell mitogens; (2) were able to stimulate allogeneic spleen cells but failed to respond in mixed lymphocyte culture; (3) failed to act as T killer cells in the CML reaction but retained their ability to kill antibody- coated target cells; and (4) did not cause graft-versus-host reaction when injected in allogeneic mice and increased their survival significantly. Furthermore, anti-MTLA was just as immunosuppressive in vivo as ATG in its ability to suppress the immune response to sheep red blood cells and prolong skin allograft survival. Anti-MTLA was found to be different in specificaties from anti-theta serum by several points. These data indicate that anti-MTLA is a specific antiserum against a unique marker on T cells distinct from the theta marker.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 10, 1974
- Accession Number
- ADA017829
Entities
People
- Aftab Ahmed
- Douglas M. Strong
- Irwin Scher
- Kenneth W. Sell
- Takenori Ochiai
Organizations
- Naval Medical Research Center