An Automated DMFB Method for the Determination of Urinary Amino Nitrogen.

Abstract

A method using 2,4-dinitrofluorobenzene (DNFB) is described for the automatic determination of urinary amino nitrogen. Based on existing manual methods, it introduces reagents that are easily adaptable to automated techniques. A mean urinary amino nitrogen value of 77.1 mg amino N/gm creatinine was obtained for a group of healthy male subjects, compared to a range of 52 to 80 mg reported by other investigators. Direct comparison of analytical results obtained by the automated method and a manual DNFB method on several urine samples yielded a mean difference of 1.34% between the methods. (Author)

Document Details

Document Type
Technical Report
Publication Date
Dec 01, 1975
Accession Number
ADA018720

Entities

People

  • Frank Bernal
  • Jesus B. Garcia Jr.

Organizations

  • United States Air Force School of Aerospace Medicine

Tags

DTIC Thesaurus Topics

  • Anatomy
  • Automatic
  • Biological Sciences
  • Body Fluids
  • Creatinine
  • Elements
  • Fluids And Secretions
  • Nitrogen
  • Urine

Readers

  • Analytical Chemistry
  • Computer Science.