Hepatic Pathology in Mice after Continuous Inhalation Exposure to 1,1,1- Trichloroethane
Abstract
Male CF-1 mice (24-34g) were exposed to either 250ppm or 1,000ppm 1, 1,1-trichloroethane in air continuously for 14 weeks. Control mice were exposed to room air. Serial sacrifice of exposed and control mice from 1 to 14 weeks demonstrated significant changes in the centrilobular hepatocytes of animals in the 1,000ppm group. Moderate liver triglyceride accumulation was evident in the 1,000ppm group and peaked at 40mg/gm of tissue (wet weight) after 7 weeks of exposure. Partial recovery was indicated by a drop in the hepatic triglyceride level to 16mg/gm by 14 weeks of exposure to 1,000ppm. Electron microscopy revealed that cytoplasmic alterations were most severe in centrilobular hepatocytes in the 1,000ppm group, and were mild to minimal in the 250ppm group. These alterations consisted of vesticulation of the rough endoplasmic reticulum with loss of attached polyribosomes, increased smooth endoplasmic reticulum, microbodies, and triglyceride droplets.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1975
- Accession Number
- ADA020124
Entities
People
- Ernest E. Mcconnell
- Fred Morris
- Neil S. Mcnutt
- Robert L. Amster
Organizations
- Air Force Research Laboratory