Resistance to Viral Challenge in the Days Immediately Following Vaccination.
Abstract
Attempts to determine how soon after vaccination there is evidence of effective protection against a challenge with homologous virsus were carried out using guinea pigs had hamsters as experimental animals. Inoculation of several group B arboviruses, Japanese encephalitis, Banzi, dengue type 2, Powassan or yellow fever, by the intraperitoneal route into guinea pigs failed to induce visible signs of illness; however, with Japanese encephalitis, Powassan, and Banzi viruses, high titered complement-fixing antibodies developed. Since no disease and death could be used as a criterion of successful infection of guinea pigs inoculated with Japanese encephalitis virus, or of hamsters inoculated with Powassan, an alternative method of determining whether early development of protection following vaccination occurred was used: development of complement-fixing antibodies at high titer was considered an indication of infection. Using this criterion it was found that following vaccination with formalin inactivated Japanese encephalitis virus, guinea pigs were protected against a homologous virus challenge from the third day after vaccination onward. Vaccination of hamsters with inactivated Powassan virus, on the other hand, failed to induce protection even when the challenge was given 7 days later.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 1975
- Accession Number
- ADA021170
Entities
People
- Jordi Casals-ariet
Organizations
- Yale University