Serum Protein-Bound Hexosamine Analysis with Autoclave Hydrolysis.
Abstract
A modification of the Winzler method for assay of serum protein-bound hexosamine is presented. The modified method eliminates the usual 4-hour hydrolysis period by substituting a 15-minute hydrolysis in an autoclave, thus allowing a more rapid processing of samples. No significant differences were found between assay values obtained with either method for a variety of samples. In addition, the concentration of the samples may be effectively doubled and the curve 'moved' to a more sensitive area of the spectrum without loss of sensitivity or accuracy.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 1976
- Accession Number
- ADA025272
Entities
People
- G. E. Routzahn
- M. J. Ryan
Organizations
- Armed Forces Radiobiology Research Institute