Biochemical, Histochemical and Electron Microscopic Study of the Subcellular Sites of Mucopolysaccharide Synthesis.
Abstract
This investigation was undertaken to develop a method for fractionation of cells secreting large amounts of sulfated mucopolysaccharides; to study the subcellular sites where intermediates metabolites and enzymes which catalyze the synthesis of sulfated mucopolysaccharides are found, and to characterize the nature of the compounds which accumulate in the Golgi apparatus during polysaccharide synthesis. Goblet cells were isolated from scrapings of exhausted colonic mucosa by trypsinizing in prsence of EDTA, followed by centrifugation in a discontinuous sucrose density gradient. The fractions obtained were examined by phase contrast and electron microscopy.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1968
- Accession Number
- ADA027636
Entities
People
- Luis R. Otero-vilardebo
Organizations
- University of Puerto Rico