The Effect of Elevated Oxygen Tension on Red Cell Function and Integrity.
Abstract
The effects of elevated oxygen tension, both hypobaric and hyperbaric, on membrane proteins and glutathione reductase of rabbit erythrocytes were investigated. Gel electrophoretic analysis revealed no discernible changes in the protein patterns of erythrocyte membranes of animals exposed to hyperoxia. Both hyperbaric and hypobaric oxygen caused a slight decrease in the glutathione reductase activity in comparison with that of control animals, but the extent of the effect was not directly proportional to the product of the duration and intensity of exposure. Rather, a threshold oxygen level was suggested. No detectable changes in erythrocyte or leukocyte counts resulted from hyperoxia. Glutathione reductase was isolated in homogeneous state from rabbit erythrocytes and was found to be a monomer with a molecular weight of 60,000. Either NADPH or NADH was capable of ruducing GSSG. The enzyme revealed substrate inhibition and was inhibited by p-hydroxymercuribenzoate under certain conditions.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1975
- Accession Number
- ADA027641
Entities
People
- John M. Prescott
- John P. Cherry
- Lee E. Ray
Organizations
- Texas A&M University