Procedures for Determination of Norepinephrine, Epinephrine, and Dopamine in Biological Specimens.
Abstract
Methods for catecholamine determination in human blood and urine have been adapted for use. Free norepinephrine (NE) in plasma was determined by trihydroxyindole (THI) fluorometric method; recoveries ranged from 71-92%, mean = 82%. Duplicate samples yielded results with standard deviation (S.D.) of + or - 37%, while replicate determinations performed on the same day varied by + or - 10%. The mean normal concentration of NE in human plasma (N = 14) was found to be 2.4 + or - 0.9 ng/ml. Concentrations of NE, epinephrine (E) and dopamine (D) were differentially determined from a uniform urine specimen by a similar THI procedure. Recoveries of NE and E ranged from 73-110%, mean = 85 + or - 16%. Day-to-day reproducibilities were + or - 16% NE and + or - 10% E. Replicate samples varied by no more than 10%. Dopamine recoveries ranged from 85-108%, with a reproducibility of + or - 8%. Normal ranges for 24-hour excretion (N = 50) were found to be 0-30 micrograms E (mean = 13), 10-80 micrograms NE (mean = 54), and 100-400 micrograms dopamine (mean = 224). (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1975
- Accession Number
- ADA028419
Entities
People
- A. B. Gustafson
- C. J. Biaselle
Organizations
- Naval Medical Research Center