Separation of Long and Short Chain Fatty Acids as Naphthacyl and Substituted Phenacyl Esters by High Performance Liquid Chromatography.
Abstract
High performance liquid chromatography of various C2 - C24 fatty acids was run on their p-bromophenacyl, p-nitrophenacyl, p-chlorophenacyl, and 2--naphthacyl esters. All the separations were accomplished using reversed phase columns with the eluent consisting of an acetonitrile:water gradient. For all derivatives tested the separations were well defined and analogous although certain esters eluted together as one peak. Quantitative results indicate that the limit of detection in the present study was two picograms of n-caproic acid and 10 picograms of arachidonic acid as their p-bromophenacyl esters.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 23, 1976
- Accession Number
- ADA029263
Entities
People
- David Rusilko
- Howard C. Jordi
Organizations
- United States Army Institute of Surgical Research