Role of Lysosomal Enzyme Release in Circulatory Shock.
Abstract
The purpose of this research project is to determine the role of lysosomal enzyme release in states of circulatory shock and conditions of critical illness. A prospective randomized double-blind study is in progress to assess the effects of a lysosomal stabilizing agent, methylprednisolone in humans in circulatory shock. A method for purifying the potent acid hydrolase, cathepsin D, has been developed in our laboratory. Infusions of purified cathepsin D into normal animals resulted in marked increases in plasma cathepsin D activity, but failed to reproduce the cardiovascular disturbances seen in shock states. In a separate study, pretreatment with a cathepsin D antiserum resulted in protection to rabbits subjected to hemorrhagic shock, presumably by binding with cathepsin D. These differing results must be resolved in future experiments. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 12, 1976
- Accession Number
- ADA030135
Entities
People
- Stephen L. Wangensteen
Organizations
- University of Virginia