Isolation and Partial Characterization of Plasma Membranes from the Livers of Control and 'Streptococcus Pneumoniae'-Infected Rats; Plasma Membranes After Pneumococcal Sepsis,
Abstract
Plasma membranes were isolated from the livers of control and Streptococcus pneumoniae-infected rats. Purity of the fractions was judged by electron microscopy and by the use of enzyme markers for microsomes (glucose-6-phosphatase), mitochondria (glutamate and malate dehydrogenase), and lysosomes (acid phosphatase). Plasma membranes from infected cells banded at the same sucrose density as plasma membranes from uninfected cells. Moreover, equivalent amounts of plasma membranes could be isolated from control and infected rat livers. There were, however, significant alterations in the enzyme complement of the plasma membrane following infection. 5'-Nucleotidase activity was significantly decreased while alkaline phosphatase activity was significantly increased. No change in the mitochondrial enzyme markers was observed following infection but the specific activity of microsomal glucose-6-phosphatase decreased significantly.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 29, 1976
- Accession Number
- ADA033390
Entities
People
- James S. Little
Organizations
- United States Army Medical Research Institute of Infectious Diseases