The Anticomplementary Activity of Lipopolysaccharide Preparations and Sonicates from 'Fusobacterium Polymorphum'.
Abstract
A complement consumption assay was employed to test the consumption of guinea pig complement (GPC') by different sonicate and lipopolysaccharide (LPS) preparations obtained from Fusobacterium polymorphum. The consumption of normal GPC' was observed at 37 C and varied depending upon the preparation used. The observed anticomplementary activity was less potent at 4 C than at 37 C. Similar anticomplementary patterns were observed when inulin, a recognized activator of the alternate complement pathway, was tested in the complement consumption assay. The reactions of a sonicate preparation of F. polymorphum with GPC' and hyperimmune rabbit antisera were not affected by the lower temperatures. The addition of heat treated (56 C and 30 min) GPC' did not enhance the complement consumption displayed by a sonicate of F. polymorphum. Further tests revealed that the factors associated with the observed anticomplementary activity were resistant to the effects of heat (100 C for 30 min) and were located in the cell wall fractions of the sonicates.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 17, 1976
- Accession Number
- ADA033904
Entities
People
- Charles E. Hawley
- William A. Falkler Jr.
Organizations
- United States Army Institute of Surgical Research