Mechanism of Resistance of 'Neisseria gonorrhoeae' to Antibiotics.
Abstract
It was found during the contract term that an inverse relationship existed between the amount of C(14)-penicillin G bound and the sensitivity of Neisseria gonorrhoeae to the antibiotic, i.e., sensitive organisms bound more benzyl penicillin than did resistant organisms. Fractionation of the whole cells indicated that binding occurred at the level of the cytoplasmic membrane and again, the inverse relationship occurred. Specificity of binding was shown by pretreatment of preparations with either unlabeled penicillin or cephalothin, in which instances binding was either strongly inhibited or abolished. From 4-9 different proteins as determined by gel electrophoresis and/or column chromatography bound proteins.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 24, 1976
- Accession Number
- ADA033964
Entities
People
- Arthur K. Saz
Organizations
- Georgetown University